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Scientist I: Assay Development

Debut Biotech

Debut Biotech

San Diego, CA, USA
Posted on Saturday, March 9, 2024

Scientist I: Assay Development


About Debut

Debut is the unconventional innovation lab spearheading the next generation of big business. Only Debut has a scalable, integrated, climate-positive creation model powered by biotechnology that puts human wellbeing at the center of everything we do—setting the new standard for profitable business and a new pace for an ever-changing world. Our hybrid biotechnology approaches unlock latent markets, providing access to sustainably produced, natural ingredients with health benefits that cannot be obtained through other methods. We are committed to traversing the entire process, from ingredient discovery to the scaling of these products, for consumer markets. If you are passionate, enjoy fast-paced innovation and collaborative teams, then Debut is for you.

Debut is growing its small scale assay team to aid in the creation of novel cell lines to create small molecules. As a Scientist in assay development you will develop, optimize, and execute novel enzymatic and cell-based assays in high-throughput screens using conventional approaches and robotics. You will also design and execute lab-based experiments, generate, and interpret data supporting the successful development of new assays and tests. As part of your day-to-day you will learn, apply, and incorporate automation solutions during assay development, execution, troubleshooting, data analysis, and reporting while investigating and identifying new methods and technologies to generate reliable and reproducible data. You will also be responsible for planning and executing experiments, adapt readily to changing project needs and develop sophisticated problem-identification and problem-solving skills.

Essential Functions

  • HTP screening of target molecules in microtiter based assays
  • Toxicity, solubility, and stability assay screening
  • Cell culture, media development, and cell-based assay development
  • Design, plan, execute, and present critical experiments in a formal setting to other key scientists
  • Coordinate with multifunctional teams for synergetic workflow
  • Mentor and lead research associates through experimental design and HTP screening processes
  • Multitasking, organization, and positive attitude is required

Education and Experience

  • PhD OR MS with 6 years experience OR BS with 8 years experience in biology, biochemistry, bioengineering, pharmacology, enzymology, or relevant field
  • Established capability to design, conduct, optimize and execute on novel biochemical and cell-based assay development efforts in the lab supporting HTS and project deliverables
  • Strong scientific foundation in the understanding and implementation of a wide range of biochemical and cell-based assay readouts for small molecule production such as HPLC, LCMS, GCMS, fluorescence Polarization, Luminescence & Fluorescence-based reporters
  • Broad experience and expertise in molecular reagent & cell line handling in aseptic environments
  • Experience with microbial fermentation at the plate and bench scale in bacteria and yeast
  • Familiarity with setting-up and operation of microplate readers and automated liquid handlers in the development of 96 well microtiter plate assays is desirable
  • Experience in computational analysis of biological data and large data sets using general-purpose programming language (such as Python, R, or Matlab) is desirable
  • Familiarity with software and statistical platforms for the analysis of large data sets (e.g., JMP, Tableau, Spotfire, or MODDE pro/DoE) is desirable
  • Ability to create and establish priorities, manage work plans, and meet timelines
  • Demonstrated initiative, teamwork, collaboration, and passion for developing technical expertise across assay development to routine execution
  • Must be detail-oriented and possess strong analytical skills
  • Must be willing to work non-standard hours supporting a 24/7 operation including weekends

Essential Physical Characteristics

The physical characteristics described here are representative of those that must be met by an employee to successfully perform the essential functions of this position. Reasonable accommodations may be made to enable qualified individuals with disabilities to perform the essential functions of a job, on a case-by-case basis.

  • Continuous upward and downward flexion of the neck.
  • Frequent: sitting, repetitive use of hands to operate computers, printers and copiers.
  • Frequently uses hands to feel objects or control tools (e.g. pipetting). Laboratory operations require dexterity and care to perform studies as required.
  • Occasional: walking, standing, climbing stairs, bending and twisting of neck, bending and twisting of waist, squatting, simple grasping, reaching above and below shoulder level, and occasional lifting and carrying of files or material weighing up to 50 pounds.
  • Must be willing to work with biohazardous agents (up to BSL2).
  • The R&D laboratory will result in exposure to hot and cold temperatures, noise, fumes, limited dust, and oily coolants.
  • The performance of this position will present exposure to an industrial environment and requires support and compliance with the published Company PPE policy including but not limited to the use of personal protective equipment such as safety glasses with side shields, appropriate attire, safety shoes, hard hat, hearing protection, etc

Condition of Employment

As part of Debut’s pre-employment process, prospective candidates will undergo a background check prior to beginning employment. Additional types of background investigations may be conducted based on the job-related activities of the position.

Debut is an equal opportunity employer. All applicants will be considered for employment without attention to race, color, religion, sex, sexual orientation, gender identity, national origin, veteran, or disability status.

Salary: $90,000 - $120,000

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